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pbi121 vector  (New England Biolabs)


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    Structured Review

    New England Biolabs pbi121 vector
    Pbi121 Vector, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 33344 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pbi121+vector/T4+DNA+Ligase/pm40138817-98-21-27
    Average 99 stars, based on 33344 article reviews
    pbi121 vector - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Plasmid Preparation:

    Article Title: Excision of DNA fragments with the <i>piggyBac</i> system in <i>Chrysanthemum morifolium</i>
    Article Snippet: .. The pBI121 vector was digested with HindIII and EcoRI, and assembled with PCR products using Gibson Assembly Master Mix (NEB, https://www.nebj.jp/). ..

    Article Title: Functions of CiHY5 in regulating the salt tolerance of Chrysanthemum revealed by transgenic Chrysanthemum indicum
    Article Snippet: .. Construction of plant expression vectors and identi cation of transgenic plants For overexpression-CiHY5 vector construction, the complete ORF sequence of CiHY5 with XbaI/XmaI sites was cloned and integrated into the pBI121 vector using T4 DNA ligase (NEB, USA), and the resulting construct was named pBI121-CiHY5 (Fig. S1). ..

    Article Title: Overexpression of an actin <i>Gh_D04G0865</i> gene in cotton reduced fineness of fiber
    Article Snippet: 1Cotton Fiber Bioscience Research Unit, United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Southern Regional Research Center (SRRC), 1100 Allen Toussaint Blvd., New Orleans, LA 70124, USA 2Cotton Structure & Quality Research Unit, Southern Regional Research Center (SRRC), Agricultural Research Service (ARS), United States Department of Agriculture (USDA), 1100 Allen Toussaint Blvd., New Orleans, LA 70124, USA

    Article Title: UDP-Api/UDP-Xyl synthases affect plant development by controlling the content of UDP-Api to regulate the RG-II-borate complex.
    Article Snippet: .. The promoter fragments were obtained via PCR amplification and subcloned into the HindIII/SmaI sites of pBI121 vector using Gibson assembly method (NEB) (Gibson et al., 2009). ..

    Article Title: A deletion/duplication in the Ligon lintless-2 locus induces siRNAs that inhibit cotton fiber cell elongation.
    Article Snippet: .. The pBI121 vector was digested with BamHI/SacI, gel purified and the cDNA of Gh_D13G2437 was inserted using HiFi DNA assembly cloning kit (New England Biolabs, Ipswich, MA, USA). ..

    Polymerase Chain Reaction:

    Article Title: Excision of DNA fragments with the &lt;i&gt;piggyBac&lt;/i&gt; system in &lt;i&gt;Chrysanthemum morifolium&lt;/i&gt;
    Article Snippet: .. The pBI121 vector was digested with HindIII and EcoRI, and assembled with PCR products using Gibson Assembly Master Mix (NEB, https://www.nebj.jp/). ..

    Article Title: Overexpression of an actin <i>Gh_D04G0865</i> gene in cotton reduced fineness of fiber
    Article Snippet: 1Cotton Fiber Bioscience Research Unit, United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Southern Regional Research Center (SRRC), 1100 Allen Toussaint Blvd., New Orleans, LA 70124, USA 2Cotton Structure & Quality Research Unit, Southern Regional Research Center (SRRC), Agricultural Research Service (ARS), United States Department of Agriculture (USDA), 1100 Allen Toussaint Blvd., New Orleans, LA 70124, USA

    Article Title: UDP-Api/UDP-Xyl synthases affect plant development by controlling the content of UDP-Api to regulate the RG-II-borate complex.
    Article Snippet: .. The promoter fragments were obtained via PCR amplification and subcloned into the HindIII/SmaI sites of pBI121 vector using Gibson assembly method (NEB) (Gibson et al., 2009). ..

    Expressing:

    Article Title: Functions of CiHY5 in regulating the salt tolerance of Chrysanthemum revealed by transgenic Chrysanthemum indicum
    Article Snippet: .. Construction of plant expression vectors and identi cation of transgenic plants For overexpression-CiHY5 vector construction, the complete ORF sequence of CiHY5 with XbaI/XmaI sites was cloned and integrated into the pBI121 vector using T4 DNA ligase (NEB, USA), and the resulting construct was named pBI121-CiHY5 (Fig. S1). ..

    Transgenic Assay:

    Article Title: Functions of CiHY5 in regulating the salt tolerance of Chrysanthemum revealed by transgenic Chrysanthemum indicum
    Article Snippet: .. Construction of plant expression vectors and identi cation of transgenic plants For overexpression-CiHY5 vector construction, the complete ORF sequence of CiHY5 with XbaI/XmaI sites was cloned and integrated into the pBI121 vector using T4 DNA ligase (NEB, USA), and the resulting construct was named pBI121-CiHY5 (Fig. S1). ..

    Over Expression:

    Article Title: Functions of CiHY5 in regulating the salt tolerance of Chrysanthemum revealed by transgenic Chrysanthemum indicum
    Article Snippet: .. Construction of plant expression vectors and identi cation of transgenic plants For overexpression-CiHY5 vector construction, the complete ORF sequence of CiHY5 with XbaI/XmaI sites was cloned and integrated into the pBI121 vector using T4 DNA ligase (NEB, USA), and the resulting construct was named pBI121-CiHY5 (Fig. S1). ..

    Article Title: Functional characterization of CiHY5 in salt tolerance of Chrysanthemum indicum and conserved role of HY5 under stress in chrysanthemum.
    Article Snippet: Among various abiotic stresses, secondary soil salinization poses a significant threat to plant productivity and survival.. Cultivated chrysanthemums (Chrysanthemum morifolium), widely grown as ornamental crops, are highly susceptible to salt stress, and their complex polyploid genome complicates the identification of stress resistance genes.. In contrast, C. indicum, a native diploid species with robust stress tolerance, serves as a valuable genetic resource for uncovering stress-responsive genes and improving the resilience of ornamental chrysanthemum cultivars.

    Sequencing:

    Article Title: Functions of CiHY5 in regulating the salt tolerance of Chrysanthemum revealed by transgenic Chrysanthemum indicum
    Article Snippet: .. Construction of plant expression vectors and identi cation of transgenic plants For overexpression-CiHY5 vector construction, the complete ORF sequence of CiHY5 with XbaI/XmaI sites was cloned and integrated into the pBI121 vector using T4 DNA ligase (NEB, USA), and the resulting construct was named pBI121-CiHY5 (Fig. S1). ..

    Article Title: Functional characterization of CiHY5 in salt tolerance of Chrysanthemum indicum and conserved role of HY5 under stress in chrysanthemum.
    Article Snippet: Among various abiotic stresses, secondary soil salinization poses a significant threat to plant productivity and survival.. Cultivated chrysanthemums (Chrysanthemum morifolium), widely grown as ornamental crops, are highly susceptible to salt stress, and their complex polyploid genome complicates the identification of stress resistance genes.. In contrast, C. indicum, a native diploid species with robust stress tolerance, serves as a valuable genetic resource for uncovering stress-responsive genes and improving the resilience of ornamental chrysanthemum cultivars.

    Clone Assay:

    Article Title: Functions of CiHY5 in regulating the salt tolerance of Chrysanthemum revealed by transgenic Chrysanthemum indicum
    Article Snippet: .. Construction of plant expression vectors and identi cation of transgenic plants For overexpression-CiHY5 vector construction, the complete ORF sequence of CiHY5 with XbaI/XmaI sites was cloned and integrated into the pBI121 vector using T4 DNA ligase (NEB, USA), and the resulting construct was named pBI121-CiHY5 (Fig. S1). ..

    Article Title: Functional characterization of CiHY5 in salt tolerance of Chrysanthemum indicum and conserved role of HY5 under stress in chrysanthemum.
    Article Snippet: Among various abiotic stresses, secondary soil salinization poses a significant threat to plant productivity and survival.. Cultivated chrysanthemums (Chrysanthemum morifolium), widely grown as ornamental crops, are highly susceptible to salt stress, and their complex polyploid genome complicates the identification of stress resistance genes.. In contrast, C. indicum, a native diploid species with robust stress tolerance, serves as a valuable genetic resource for uncovering stress-responsive genes and improving the resilience of ornamental chrysanthemum cultivars.

    Article Title: Shoot-to-root mobile CEPD-like 2 integrates shoot nitrogen status to systemically regulate nitrate uptake in Arabidopsis
    Article Snippet: .. We cloned the CEPDL1 or CEPDL2 ORFs into Xba I-, Sac I-digested pBI121 vector downstream of the CaMV 35 S promoter using the Gibson Assembly system (New England Biolabs). .. General sequence analysis was performed using GENETYX-MAC software (Genetyx, Tokyo, Japan).

    Construct:

    Article Title: Functions of CiHY5 in regulating the salt tolerance of Chrysanthemum revealed by transgenic Chrysanthemum indicum
    Article Snippet: .. Construction of plant expression vectors and identi cation of transgenic plants For overexpression-CiHY5 vector construction, the complete ORF sequence of CiHY5 with XbaI/XmaI sites was cloned and integrated into the pBI121 vector using T4 DNA ligase (NEB, USA), and the resulting construct was named pBI121-CiHY5 (Fig. S1). ..

    Article Title: Functional characterization of CiHY5 in salt tolerance of Chrysanthemum indicum and conserved role of HY5 under stress in chrysanthemum.
    Article Snippet: Among various abiotic stresses, secondary soil salinization poses a significant threat to plant productivity and survival.. Cultivated chrysanthemums (Chrysanthemum morifolium), widely grown as ornamental crops, are highly susceptible to salt stress, and their complex polyploid genome complicates the identification of stress resistance genes.. In contrast, C. indicum, a native diploid species with robust stress tolerance, serves as a valuable genetic resource for uncovering stress-responsive genes and improving the resilience of ornamental chrysanthemum cultivars.

    Purification:

    Article Title: Overexpression of an actin <i>Gh_D04G0865</i> gene in cotton reduced fineness of fiber
    Article Snippet: 1Cotton Fiber Bioscience Research Unit, United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Southern Regional Research Center (SRRC), 1100 Allen Toussaint Blvd., New Orleans, LA 70124, USA 2Cotton Structure & Quality Research Unit, Southern Regional Research Center (SRRC), Agricultural Research Service (ARS), United States Department of Agriculture (USDA), 1100 Allen Toussaint Blvd., New Orleans, LA 70124, USA

    Article Title: A deletion/duplication in the Ligon lintless-2 locus induces siRNAs that inhibit cotton fiber cell elongation.
    Article Snippet: .. The pBI121 vector was digested with BamHI/SacI, gel purified and the cDNA of Gh_D13G2437 was inserted using HiFi DNA assembly cloning kit (New England Biolabs, Ipswich, MA, USA). ..

    Amplification:

    Article Title: Overexpression of an actin <i>Gh_D04G0865</i> gene in cotton reduced fineness of fiber
    Article Snippet: 1Cotton Fiber Bioscience Research Unit, United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Southern Regional Research Center (SRRC), 1100 Allen Toussaint Blvd., New Orleans, LA 70124, USA 2Cotton Structure & Quality Research Unit, Southern Regional Research Center (SRRC), Agricultural Research Service (ARS), United States Department of Agriculture (USDA), 1100 Allen Toussaint Blvd., New Orleans, LA 70124, USA

    Article Title: UDP-Api/UDP-Xyl synthases affect plant development by controlling the content of UDP-Api to regulate the RG-II-borate complex.
    Article Snippet: .. The promoter fragments were obtained via PCR amplification and subcloned into the HindIII/SmaI sites of pBI121 vector using Gibson assembly method (NEB) (Gibson et al., 2009). ..

    Cloning:

    Article Title: Overexpression of an actin <i>Gh_D04G0865</i> gene in cotton reduced fineness of fiber
    Article Snippet: 1Cotton Fiber Bioscience Research Unit, United States Department of Agriculture (USDA), Agricultural Research Service (ARS), Southern Regional Research Center (SRRC), 1100 Allen Toussaint Blvd., New Orleans, LA 70124, USA 2Cotton Structure & Quality Research Unit, Southern Regional Research Center (SRRC), Agricultural Research Service (ARS), United States Department of Agriculture (USDA), 1100 Allen Toussaint Blvd., New Orleans, LA 70124, USA

    Article Title: A deletion/duplication in the Ligon lintless-2 locus induces siRNAs that inhibit cotton fiber cell elongation.
    Article Snippet: .. The pBI121 vector was digested with BamHI/SacI, gel purified and the cDNA of Gh_D13G2437 was inserted using HiFi DNA assembly cloning kit (New England Biolabs, Ipswich, MA, USA). ..



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